Cryo-Ultramicrotomy of Complex Molecular Fluids
نویسندگان
چکیده
منابع مشابه
Cryo-ultramicrotomy of islets of Langerhans. Some observations on the fine structure of mammalian islets in frozen sections.
A procedure is described for the preparation of ultrathin frozen sections of glutaraldehydefixed or unfixed islets of Langerhans by cryo-ultramicrotomy. Freezing of the tissue was accomplished by direct immersion of isolated islets in liquid nitrogen. Sectioning was performed at a specimen temperature of —80 °C and a knife temperature of —40 °C, the ribbon of sections being collected on a troug...
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The feasibility of using a focused ion beam (FIB) for the purpose of thinning vitreously frozen biological specimens for transmission electron microscopy (TEM) was explored. A concern was whether heat transfer beyond the direct ion interaction layer might devitrify the ice. To test this possibility, we milled vitreously frozen water on a standard TEM grid with a 30-keV Ga(+) beam, and cryo-tran...
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Microfluidics is very important in the world of BioMEMS, particularly for lab-ona-chip devices. There are many unusual differences from conventional fluidics, such as the significance of surface forces, the high shear/extensional rate, the high heat transfer rate, the low Reynolds number, and the high Weissenberg number. In this study, aqueous solutions of high molecular-weight polymers, polyet...
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ژورنال
عنوان ژورنال: Microscopy Today
سال: 2018
ISSN: 1551-9295,2150-3583
DOI: 10.1017/s1551929517001390